Identifying the association between polymorphisms in the GZMB and IFIH1 genes and psoriasis in Egyptians

ABSTRACT

Objectives

This study aims to examine whether the genetic variants in the genes for Granzyme B (GZMB) and Interferon Induced with Helicase C domain 1 (IFIH1) were associated with psoriasis.

Background

Psoriasis, a papulosquamous skin disease, was initially thought of as a disorder primarily of epidermal keratinocytes but is now recognized as one of the most common immune-mediated disorders. It is caused by the interplay between multiple genetic and environmental risk factors.

Subjects and methods

This case-control study has 65 participants with psoriasis and 65 healthy controls. Real-time PCR was used to genotype GZMB (rs8192917) and IFIH1 (rs35667974).

Results

Genotype occurrence and allelic spreading for both SNPs are in Hardy – Weinberg equilibrium. The genotype and allele distributions of rs35667974 showed no differences between the studied groups. Regarding rs8192917, compared to Group II, there is a statistically significant rise in the CC genotype and C allele in Group I. Higher PASI scores are detected in the C/C and C/T genotypes more than the T/T genotype. Univariate and multivariate analyses revealed that BMI, catalase, MDA, and rs8192917 (C/C) are associated with psoriasis.

Conclusion

GZMB rs8192917 was significantly related to psoriasis risk; its C allele is likewise associated with psoriasis vulnerability. However, our investigation found no link between rs35667974 and psoriasis.

KEYWORDS:

• Psoriasis
• SNP
• GZMB
• IFIH1
• real time-PCR

Disclosure statement

No potential conflict of interest was reported by the author(s).

Authors’ contribution statement

Sara Kamal Rizk: conceptualization (lead), writing – original draft, visualization, and investigation. Azza Gaber: review and editing, formal analysis, and visualization. Samah El-Ghlban: review and editing (equal), and visualization. Israa Salah Eldin: methodology, writing – review and editing (equal).

Data sharing statement

Data is available upon reasonable request from the corresponding author.

Additional information

Funding

No funds.