Low frequency vortex magnetic field reduces amyloid β aggregation, increase cell viability and protect from amyloid β toxicity

ABSTRACT

Plaques formed by abnormal accumulation of amyloid β-peptide (Aβ) lead to onset of Alzheimer’s disease (AD). Pharmacological treatments do not reduce Aβ aggregation neither restore learning and memory. Noninvasive techniques have emerged as an alternative to treat AD, such as stimulation with electromagnetic fields (EMF) that decrease Aβ deposition and reverses cognitive impairment in AD mice, even though some studies showed side effects on parallel magnetic fields stimulation. As a new approach of magnetic field (MF) stimulation, vortex magnetic fields (VMF) have been tested inducing a random movement of charged biomolecules in cells, promoting cell viability and apparently safer than parallel magnetic fields. In this study we demonstrate the effect of VMF on Aβ aggregation. The experimental strategy includes, i) design and construction of a coil capable to induce VMF, ii) evaluation of VMF stimulation on Aβ peptide induced-fibrils-formation, iii) evaluation of VMF stimulation on SH-SY5Y neuroblastoma cell line in the presence of Aβ peptide. We demonstrated for the first time that Aβ aggregation exposed to VMF during 24 h decreased ~ 86% of Aβ fibril formation compared to control. Likewise, VMF stimulation reduced Aβ fibrils-cytotoxicity and increase SH-SY5Y cell viability. These data establish the basis for future investigation that involve VMF as inhibitor of Aβ-pathology and indicate the therapeutic potential of VMF for AD treatment.

KEYWORDS:

• Thioflavin-t assay
• magnetic stimulation
• amyloid β plaques
• Alzheimer’s disease
• Rodin coil

Acknowledgments

The authors thankfully acknowledge Diana Aparicio-Bautista PhD and Daniel Chavez Valenzuela PhD for their technical support.

Disclosure statement

There are no conflicts of interest to declare.

Additional information

Funding

This work was supported by CONACyT under [Grant no. A1-S-29906]; Convocatoria Institucional de Investigación Científica 2020, Universidad de Guanajuato under [project 065/2020].