Comparison of effects of tobacco cigarettes, electronic nicotine delivery systems and tobacco heating products on miRNA-mediated gene expression. A systematic review

Abstract

Objectives

This work attempts to summarize current knowledge on the effects of cigarettes, electronic nicotine delivery systems and tobacco heating products on miRNA-mediated gene expression regulation and on their possible impact on smoking-related respiratory disease development.

Materials and methods

Literature search by terms combination: ’smoking’, ‘cigarette’ ‘THP’, ‘tobacco heating product’, ‘ENDS’, ‘electronic nicotine delivery system’, ‘e-cigarette’, electronic cigarette’ and ‘miRNA-mediated gene expression’ has been performed from October 2021 to February 2022. In this systematic review all relevant literature, including clinical trials, cellular and animal-based studies were included.

Results

Cigarette smoke (CS) significantly altered transcriptome, including miRNAs expression profile. MiRNA-mediated gene expression is mentioned as one of the mechanisms associated with smoking-related respiratory disease development. Differential expression of miRNAs was reduced in aerosol from e-cigarettes (EC) and tobacco heating products (THP) when compared to CS. However, there was a significant alteration of some miRNAs expression when compared to air-controls in both EC and THP.

Discussion

CS negatively affects transcriptome and miRNA-mediated gene expression regulation because of a huge number of hazardous substances which predispose to smoking-related diseases. Despite the reduced effect of ENDS and THP on miRNAs profile compared to CS, differences in expression of miRNAs when compared to air-control were observed, which may be harmful to never-smokers who may perceive such alternative smoking products as non-hazardous. To clearly indicate the role of ENDS and THP in the alteration of miRNA-mediated gene expression and the development of smoking-related respiratory diseases associated with this mechanism, more long-term studies should be performed in the future.

Keywords:

• Smoking
• miRNA
• gene expression
• electronic nicotine delivery system
• tobacco heating product

Disclosure statement

The authors report no conflict of interest. The authors report no declaration of interest.

Additional information

Funding

This work was supported by a Medical University of Lodz Grant: 503/0-149-03/503-01-001-19-00.