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ABSTRACT
Cyclin proteins are the key regulatory and activity partner of cyclin-dependent kinases (CDKs), which play pivotal regulatory roles in cell cycle progression. In the present study, we identified a Cyclin L1 and 2 CDK11 2 CDK11 splice variants, CDK11A and CDK11B, from silkworm, Bombyx mori. We determined that both Cyclin L1 and CDK11A/B are nuclear proteins, and further investigations were conducted to elucidate their spatiofunctional features. Cyclin L1 forms a complex with CDK11A/B and were co-localized to the nucleus. Moreover, the dimerization of CDK11A and CDK11B and the effects of Cyclin L1 and CDK11A/B on cell cycle regulation were also investigated. Using overexpression or RNA interference experiments, we demonstrated that the abnormal expression of Cyclin L1 and CDK11A/B leads to cell cycle arrest and cell proliferation suppression. Together, these findings indicate that CDK11A/B interacts with Cyclin L1 to regulate the cell cycle.
KEYWORDS:
Disclosure of potential conflicts of interest
The authors declare that they have no competing financial interests.
Acknowledgments
We thank Dr. Xiao-lin Zhou for the help in performing the experiments. The National Natural Science Foundation of China (Grant Nos. 31272505, 31472152, and 31572466) and China Agriculture Research System (CARS-22) supported this study.
Author contributions statement
Conceived and designed the experiments: Tai-hang Liu, Yun-fei Wu, and Xiao-long Dong. Performed the experiments performance: Tai-hang Liu, Yun-fei Wu, Xiao-long Dong, Cai-xia Pan and Guo-yu Du. Data analysis: Tai-hang Liu, Ji-gui Yang, Wei Wang and Xi-yan Bao. Reagents/materials/analysis tools contribution: Peng Chen, Min-hui Pan and Cheng Lu. Paper written: Tai-hang Liu and Yun-fei Wu.
