![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
ABSTRACT
Long non-coding RNAs (LncRNAs) have been recently regarded as systemic regulators in multiple biologic processes including tumorigenesis. In this study, we observed the expression of lncRNA lnc-sox5 was significantly increased in colorectal cancer (CRC). Despite the CRC cell growth, cell cycle and cell apoptosis was not affected by lnc-sox5 knock-down, lnc-sox5 knock-down suppressed CRC cell migration and invasion. In addition, xenograft animal model suggested that lnc-sox5 knock-down significantly suppressed the CRC tumorigenesis. Our results also showed that the expression of indoleamine 2,3-dioxygenase 1 (IDO1) was significantly reduced by lnc-sox5 knock-down and therefore modulated the infiltration and cytotoxicity of CD3+CD8+T cells. Taken together, these results suggested that lnc-sox5 unbalances tumor microenvironment to regulate colorectal cancer progression.
Disclosure of potential conflicts of interest
No potential conflicts of interest were disclosed.
Funding
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Author contributions
Conceived and supervised the study by Liang Wang from Gastrointestinal Surgery Center, 1st Affiliated Hospital of Sun Yat-Sen University. The cell lines are constructed by Zhenxian Zhao from Department of Pancreato-Biliary Surgery, 1st Affiliated Hospital of Sun Yat-Sen University. The construction of animal model, flow cytometry analysis and the manuscript writing is performed by Kaiming Wu from Gastrointestinal Surgery Center, 1st Affiliated Hospital of Sun Yat-Sen University. We also thank Kuanzhi Liu for the animal grooming, Jian Zhang for the Western blot assay, Guanghua Li for the cell culture and manuscript checking.
