Fluorescence Turn-On Sensing of Ascorbic Acid Based on a Hyperbranched Conjugated Polyelectrolyte

Abstract

A fluorescent turn-on detection for ascorbic acid has been developed based on hyperbranched conjugated polyelectrolyte. The fluorescence probe benefits from the high signal amplification from hyperbranched molecular structure of the conjugated polymer and produce fluorescence superquenching by Cu²⁺ ions with a detection limit down to the nanomolar range. Upon addition of ascorbic acid, the paramagnetic Cu²⁺ ions can be transformed into diamagnetic Cu¹⁺ ions, which inhibit the quenching and thus lead to fluorescence enhancement of the hyperbranched conjugate polymer. Other biologically relevant species, such as glucose, uric acid, L-arginine, and acetaminophen do not lead to significant fluorescence change of the conjugated polymer-Cu²⁺ complex, indicating that the hyperbranched conjugated polymer-Cu²⁺ complex can thus serve as an effective chemosensor to detect ascorbic acid. [Supplementary materials are available for this article. Go to the publisher's online edition of Soft Materials for the following free supplemental resource(s): Supporting Information]

Keywords:

• Ascorbic acid
• Conjugated polyelectrolyte
• Sensor

Acknowledgments

This work was financially supported by the National Basic Research Program of China (2012CB933301 and 2009CB930600), the National Natural Science Foundation of China (90813010 and 2120438), the Ministry of Education of China (IRT1148), and the National Science Foundation of Jiangsu Provence (11KJB150010, NY 211023, and NY 211047).