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Research Article

In-silico modeling of early-stage biofilm formation

, , , , & ORCID Icon
Pages 346-358 | Received 30 Oct 2020, Accepted 07 Jan 2021, Published online: 15 Mar 2021
 

ABSTRACT

Bacteria can form biofilms in different environmental conditions, e.g. pH, temperature, nutrients, etc. Biofilm growth, therefore, is an extremely robust process. Because of this, while biofilm growth is a complex process affected by several variables, insights into biofilm formation could be obtained by studying simple schematic models. In this manuscript, we describe a hybrid molecular dynamics and Monte Carlo model for the simulation of the early stage formation of a biofilm, to explicitly demonstrate that it is possible to account for most of the processes expected to be relevant. The simulations account for the growth and reproduction of the bacteria, for their interaction and motility, for the synthesis of extracellular polymeric substances and exopolisaccharide trails. We describe the effect of these processes on the early stage formation of biofilms, in two dimensions, and also discuss preliminary three-dimensional results.

Acknowledgments

N.P. and M.P.C. acknowledge support from the Singapore Ministry of Education through the Academic Research Fund MOE2017-T2-1-066 (S), and are grateful to the National Supercomputing Centre (NSCC) for providing computational resources.

Additional information

Funding

This work was supported by the Ministry of Education - Singapore [2019-T1-001-03].

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