Nucleic Acids Isolation for Molecular Diagnostics: Present and Future of the Silica-based DNA/RNA Purification Technologies

ABSTRACT

At present, most commercial kits for nucleic acid isolation are silica-based. In this regard, new strategies are under development with the aim of optimizing nucleic acid purification silica-based protocols. This review aims to summarize the evolution of such strategies and highlight the current necessities to be solved by new purification methods based on silica materials. In addition, it will perform a survey of the purified DNA/RNA quality and quantity for a subsequent technique. This information is of vital importance to consider for the success of molecular diagnostics. The text was designed to guide scientists and engineers in choosing suitable DNA/RNA purification techniques for research or bioprocessing applications.

KEYWORDS:

• Silica
• purification
• nucleic-acids
• diagnostics

Acknowledgments

MG and MLS are researchers from CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas) (IMBICE-CONICET). IAR, GR and PV acknowledge the Consejo Nacional de Ciencia y Tecnología (CONACYT scholarship 966895, 1048769 and 886264). JB is a researcher from ITESM.

AUTHOR CONTRIBUTIONS

Sánchez, Mirna L.: Conceptualization, supervision, writing, review & editing. Peñaloza Coronas, Cynthia: Investigation, writing, editing. Avila Rodríguez, Maria Isabela: Investigation, writing, editing. Viaña Mendieta, Pamela: Investigation, writing, editing. Rivera Hernández, Gabriela: Investigation, writing, editing. Benavides, Jorge: review. Grasselli, Mariano: writing, review & editing.

ETHICS APPROVAL

No ethical approval or consent was required for the present article.

DISCLOSURE STATEMENT

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was financially supported by grants from Consejo Nacional de Ciencia y Tecnología 886264, 966895, 1048769 (CONACYT (886264, 966895 and 1048769).