IGF-1 Gene Polymorphism and its Association with Cashmere Fiber Trait in Changthangi Goats

ABSTRACT

The Cashmere (Pashmina) fiber attracts great demand worldwide for its fineness. To identify and determine the role of Insulin-like Growth Factor-1 (IGF-1) gene polymorphism on Pashmina fiber traits (quality and quantity), exon IV, and part of intron IV of the IGF-1 gene of Changthangi goats were analyzed. 103 Changthangi goats of either sex were divided into two groups, the high producers and low producers of Pashmina. Upon restriction digestion with HaeIII, three genotypes were identified, i.e., A₁A₁ (0.253), A₂A₂ (0.320), and A₁A₂ (0.427). These genotypes did not vary significantly (p > .05) from Hardy–Weinberg Equilibrium. DNA sequence analysis revealed G > C transversion in intron-IV of IGF-1 gene at the HaeIII restriction site. The SNP effect of two alleles on mean Cashmere fiber diameter of down fibers (MFD) was statistically significant (p < .05) and the average finest fiber diameter was 13.46 µ for genotype A₂A_2. The mean genetic variability parameters for the gene analyzed were heterozygosity value (0.4977), PIC Value (0.3738), Effective allele no (1.9908),and Shannon index I (0.6908). The significant association of IGF-1 gene polymorphism and fiber finesse can be exploited in breeding programmes to improve the production and quality of Pashmina fiber in goat populations.

摘要

羊绒纤维（pashmina）的细度在世界范围内引起了极大的需求.为了鉴定和确定胰岛素样生长因子1（IGF-1）多态性在山羊Pashmina纤维性状（品质和数量）中的作用，对Changthangi山羊IGF-1基因的外显子IV和部分内含子IV进行了分析.103只不同性别的Changthangi山羊被分为两组，分别是Pashmina的高产量山羊和低产量山羊.经限制性分析，鉴定出A1A1（0.253）、A2A2（0.320）和A1A2（0.427）三个基因型.这些基因型与HardyWeinberg平衡无显著差异（P>0.05）。DNA序列分析显示，在hEIII限制位点，IGF-1基因的内含子IV存在G>C的交叉.Changthangi山羊2个等位基因对绒纤维平均直径（mfd）的单核苷酸多态性影响具有统计学意义（P<0.05），A2A2基因型绒纤维平均直径为13.46μ.所分析基因的平均遗传变异参数为杂合度值（0.4977）、PIC值（0.3738）、有效等位基因（1.9908）和香农指数I（0.6908）.IGF1基因多态性和纤维精细度的显著关联可以在育种计划中加以利用，以提高山羊群体中Pashmina纤维的产量和质量.

KEYWORDS:

• Association
• changthangi
• IGF
• pashmina
• polymorphism
• sequence

关键词:

• 关键词
• 协会
• 多态性
• 序列

Conflict of interest

The authors declare that they have no conflict of interest.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Notes on contributors

Syed Shanaz

Syed Shanaz, Division of Animal Genetics and Breeding, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shuhama, Alusteng, Srinagar, Kashmir, J&K-190006, India.

Tasleem Ahmad Shamas Ganai

Tasleem Ahmad Shamas Ganai, Directorate of Education, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, J&K, India.

Ishraq Hussain

Ishraq Hussain, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shuhama, Alusteng, Srinagar, Kashmir, J&K-190006, India.

Sheikh Firdous Ahmad

Sheikh Firdous Ahmad, Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP -243122, India.

Feroz Din Sheikh

Feroz Din Sheikh, Division of Animal Genetics and Breeding, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shuhama, Alusteng, Srinagar, Kashmir, J&K-190006, India.

Nadeem Shabir

Nadeem Shabir, Division of Animal Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shuhama, Alusteng, Srinagar, Kashmir, J&K-190006, India.

Nusrat Nabi

Nusrat Nabi, Division of Animal Genetics and Breeding, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shuhama, Alusteng, Srinagar, Kashmir, J&K-190006, India.

Nazir A Ganai

Nazir A Ganai, Division of Animal Genetics and Breeding, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shuhama, Alusteng, Srinagar, Kashmir, J&K-190006, India.