Study of Wound Dressing Material Coated with Natural Extracts of Calotropis Gigantean, Eucalyptus Globulus and Buds of Syzygium Aromaticum Solution Enhanced with rhEGF (REGEN-D^TM 60)

ABSTRACT

In this work, the leave extracts of Calotropis gigantean, Eucalyptus globulus and buds of Syzygium aromaticum were prepared and coated on natural fibers based bamboo gauze fabric for wound dressing applications. The fabrics coated with different compositions of the natural extract were enhanced with rhEGF (REGEN-D™ 60). The morphology of developed samples was studied using SEM to know its pores sizes. Various mechanical properties have been studied. The antibacterial activity was studied using Gram positive and negative types of bacteria. The wound healing efficacy of the coated fabric samples were studied through in vivo method. The type of wound selected for the study was second degree wound. The dorsal surface of the rat’s skin was removed and second degree wound was created. Then the developed fabric samples were dressed over the wound surface and the wound healing was studied by wound closure rate in equal interval of days. A specified composition of natural extracts coated on bamboo gauze fabric enhanced with rhEGF (REGEN-D™ 60) exhibits good antibacterial property was confirmed from the formation of zone of inhibition in antibacterial test against Escherichia coli and a Staphylococcus aureus and appropriate wound closure rate on second degree wound through in vivo evaluation.

摘要

本研究以天然纤维为基础的竹纱织物为敷料，制备了Calotropis gigantean叶提取物、桉树球提取物和Syzygium aromaticum芽提取物. 用rhEGF（REGEN-D™60）对不同成分的天然提取物涂层织物进行增强处理. 利用扫描电镜（SEM）对所制备样品的形貌进行了研究，以确定其孔尺寸. 研究了各种力学性能. 用革兰氏阳性菌和阴性菌进行抗菌活性研究. 通过体内实验研究了涂层织物的创面愈合效果. 为研究选择的伤口类型是二级伤口. 切除大鼠皮肤背表面，造成二度创伤. 然后将研制好的织物样品覆盖在创面上，用等间隔时间的创面闭合率研究创面愈合情况. 通过对大肠埃希菌和金黄色葡萄球菌的抗菌试验中抑菌区的形成和二度伤口的适当闭合率，确定了用rhEGF（REGEN-D™60）增强竹纱织物表面天然提取物的抗菌性能活体评估.

KEYWORDS:

• Anti-bacterial
• bamboo fiber
• leaves
• natural extract
• wound dressing
• wound healing

关键词:

• 抗菌
• 竹纤维
• 离开
• 天然提取物
• 伤口敷料
• 伤口愈合