Modified Nodal Cuttings and Shoot Tips Protocol for Rapid Regeneration of Cannabis sativa L

ABSTRACT

The aim of this study was to obtain micropropagation protocol for fibrous, Cannabidiol (CBD) rich, hemp variety Epsilon 68. To fulfil this goal, nodal and tip cutting protocol was developed. In further step, this protocol was compared with several other methods dependent on plant growth regulators with cytokine activity to obtain 4.2 ± 1.43 SD explants per tip. Plants from isolated lines were acclimatized to field conditions, and the content of cannabinoids was evaluated in the inflorescences. Described micropropagation protocol may be useful in breeding of hemp varieties and germplasm conservation.

摘要

本研究的目的是获得富含纤维、CBD(大麻二酚)、大麻品种Epsilon 68的微繁方案. 为了实现这一目标，制定了节点和尖端切割方案. 在进一步的步骤中，我们将该方案与其他几种依赖于具有细胞因子活性的植物生长调节剂的方法进行了比较. 方法:取4.2±1.43 SD的外植体. 对离体植株进行了田间驯化，测定了花序中大麻素的含量. 该方法可用于大麻品种的选育和种质资源的保护.

KEYWORDS:

• Hemp
• micropropagation
• plant regeneration
• cannabinoids

关键词:

• 麻
• 微体繁殖
• 植物再生
• 大麻类

Acknowledgments

We thank Ms. Marta Kaczmarek for assistance with preparation of Figure 2.

Additional information

Funding

This work was financed by the National Centre for Research and Development, grant number INNOMED/I/11/NCBR/2014.