Screening and Selection of Drought-resistant Aegilops Triuncialis Accessions by Physiological and Molecular Markers

ABSTRACT

The wheat straw fibers are cheap, easily attainable and environmentally friendly. However, the plant is susceptible to different biotic and abiotic stresses. Crop wild relatives (CWRs) are a suitable source of different characters such as tolerance to abiotic and biotic stresses, for plant breeding. This study elucidates the association between DHNs and Dreb1 genes and physiological traits such as proline and relative water content (RWC) as well as some drought tolerance indices for identifying resistant Aegilops triuncialis populations to drought stress. Our results showed that populations collected from dry regions (Karaj and Tabriz) had higher proline, RWC and seed yield compared to other populations under drought stress. Also, the results revealed that the DHN15 primer produced a high level of genetic polymorphism compared to the other DHNs primers. According to the results of DHNs primers, the Ilam population was genetically diverse from the other populations. The present study demonstrated that the polymorphism revealed by the Dreb1 primer was higher than DHN primers. Therefore, the Dreb1 primer was more effective to distinguish drought-resistant Ae. triuncialis populations. Based on physiological and molecular analyses, Karaj and Tabriz populations were drought-resistant. Our finding suggested that further research on A. triuncialis would help pyramid the genetic traits to improve drought tolerance in common wheat.

摘要

麦秸纤维价格便宜,易得,环保. 然而, 这种植物容易受到不同的生物和非生物胁迫. 作物野生近亲 (CWR) 是植物育种的一个合适来源, 具有不同的特性, 如对非生物和生物胁迫的耐受性. 本研究阐明了DHNs和Dreb1基因与脯氨酸和相对含水量 (RWC) 等生理性状以及一些耐旱性指标之间的关联, 以确定三钩山羊草种群对干旱胁迫的抗性. 我们的结果表明, 与干旱胁迫下的其他种群相比, 干旱地区 (卡拉吉和大不里士) 的种群具有更高的脯氨酸,RWC和种子产量. 此外, 结果显示, 与其他DHNs引物相比, DHN15引物产生了高水平的遗传多态性. 根据DHNs引物的结果, Ilam群体在遗传上与其他群体不同. 本研究表明, Dreb1引物显示的多态性高于DHN引物. 因此, Dreb1引物能更有效地识别抗旱性Ae. 三叉戟种群. 根据生理和分子分析, Karaj和Tabriz种群具有抗旱性. 我们的发现表明, 对三叉戟天牛的进一步研究将有助于提高普通小麦的抗旱性.

KEYWORDS:

• RWC
• proline
• drought stress
• function markers
• wheat
• Aegilops

关键词:

• 脯氨酸
• 干旱胁迫
• 功能标记
• 小麦
• 山羊草

Disclosure statement

No potential conflict of interest was reported by the author(s).