Effect of Ultrasonic Pretreatment on the Biosynthesis of Tocopherols, Tocotrienols and Carotenoids in Flax Sprouts (Linum Usitatissimum L.)

ABSTRACT

This study evaluated the effect of ultrasonic pretreatment of flaxseeds for 0–10 min on the biosynthesis of tocopherols, tocotrienols, and carotenoids in flax sprouts. The results showed that tocopherol, tocotrienol and carotenoid contents were significantly increased (up to 130%) after 8-min ultrasonic pretreatment in LT001 flax sprouts as compared with control group. The similar increasing trend of tocopherols and tocotrienols was occurred in fiber flax sprouts after ultrasonic pretreatment, while carotenoid contents showed a decreasing trend. The expression levels of genes involved in the biosynthesis of tocopherols, tocotrienols, and carotenoids were analyzed and the results could partly explain the changing trends of tocopherols, tocotrienols and carotenoids in two different varieties of flax sprouts responding to ultrasonic pretreatments. Present study could be useful guidance for ultrasonic pretreatment to enhance micronutrients and biofortify nutritional values in flax sprouts.

摘要

本研究评估了超声波预处理亚麻籽0-10分钟对亚麻芽中生育酚、 生育三烯酚和类胡萝卜素生物合成的影响。结果表明，与对照组相比，LT001亚麻芽经8min超声波预处理后，生育酚、 生育三烯酚和类胡萝卜素含量显著增加 (高达130%)。 纤维亚麻芽经超声波预处理后, 生育酚和生育三烯酚的含量呈相似的上升趋势，类胡萝卜素含量呈下降趋势。分析了生育酚、生育三烯酚和类胡萝卜素生物合成相关基因的表达水平，结果部分解释了超声波预处理后两种不同亚麻芽中生育酚、生育三烯酚和类胡萝卜素的变化趋势。本研究为超声波预处理提高亚麻芽中微量元素含量和生物强化营养价值提供了有益的指导。

KEYWORDS:

• Flax sprouts
• ultrasonic pretreatment
• tocopherols
• tocotrienols
• carotenoids
• biosynthesis

关键词:

• 亚麻芽
• 超声波预处理
• 生育酚
• 生育三烯酚
• 类胡萝卜素
• 生物合成

HIGHLIGHT

• Ultrasonic pretreatment could enhance tocopherols, tocotrienols, and carotenoids in flax sprouts.

• α- and β- tocopherols were significantly raised with ultrasonic pretreatment.

• Lutein was the main carotenoid compound in flax sprouts and sensitive to ultrasonic pretreatment.

• LT001 flax was more sensitive to ultrasonic pretreatment than Zhongya 4 flax in sprouts.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was funded by National Natural Science Foundation of Guangdong Province (2021A1515012110), Central Public-interest Scientific Institution Research Fund (1610242021001), Agricultural Science and Technology Innovation Program in Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2017-IBFC06) and China Agriculture Research System of MOF and MARA for financial support.