Horseradish Peroxidase-Catalyzed Coloration of Natural Phenols on Silk Fabric: Effects of Various Buffer Systems and pH Value on Dyed Color

ABSTRACT

Currently, the known possible shades obtained for coloring silk fabrics using enzyme catalysts such as peroxidase are limited to a few shades of brownish tint. For the oxidative dyeing method using horseradish peroxidase (HRP) to become common, it must be able to produce a more diverse range of colors; this is a problem that must be solved. The purpose of this study is to obtain various colors by dyeing natural phenolic substrates using HRP in various buffers (acetate, citrate, phosphate buffer) and various pH levels (3 ~ 9). We emphasized color diversity, which was achieved through the exploration of six different natural aromatic compounds: gallic acid (GA), guaiacol (GAC), p-coumalic acid (CA), ferulic acid (FA), catechol (CAC) and caffeic acid (CFA). We also performed a methodological survey of reaction processing parameters. The colored products generated through HRP catalysis were analyzed using UV-vis. The color of the dyed fabrics was analyzed using the CIE L*a*b* color space system. As a result of the experiment, we obtained silk fabrics with various color depths from each buffer system. This suggests that the desired color and tone of dyed silk fabrics can be achieved by adjusting the buffer system and pH value.

摘要

目前, 已知的使用过氧化物酶等酶催化剂为丝绸着色的可能色度仅限于几种棕色. 为了使使用辣根过氧化物酶(HRP)的氧化染色方法变得普遍, 它必须能够产生更多样化的颜色;这是一个必须解决的问题. 本研究的目的是在不同的缓冲液(醋酸盐、柠檬酸盐、磷酸盐缓冲液)和不同的pH值(3~90)下, 使用HRP对天然酚基质进行染色, 以获得不同的颜色. 我们强调颜色多样性, 这是通过探索六种不同的天然芳香化合物实现的:没食子酸(GA)、愈创木酚(GAC)、对香豆酸(CA)、阿魏酸(FA)、邻苯二酚(CAC)和咖啡酸(CFA). 我们还对反应过程参数进行了方法学调查. 用紫外-可见光谱法对HRP催化生成的有色产物进行了分析. 使用CIE L*a*b*颜色空间系统分析染色织物的颜色. 作为实验结果, 我们从每个缓冲系统中获得了具有不同颜色深度的真丝织物. 这表明, 通过调节缓冲体系和pH值, 可以获得所需的染色真丝织物的颜色和色调.

KEYWORDS:

• Enzymatic dyeing
• horseradish peroxidase
• coloration
• various buffer system
• natural dyes
• silk

关键词:

• 酶染色
• 辣根过氧化物酶
• 自然色彩
• 各种缓冲系统
• 天然染料
• 蚕丝

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [21674043].