Association of MTHFR C677T Polymorphism with Preeclampsia in North East of Iran (Khorasan Province)

Abstract

Background: Preeclampsia (PE) is one of the main causes of fetal and maternal mortality. The analysis of candidate gene polymorphisms can improve our understanding of the mechanisms underlying pathogenesis of PE. Present study is aimed at investigating the association between MTRR c.66A > G, MTHFR c.677C > T, MTHFR c.1298A > C, and MTR c.2756A > G polymorphisms and PE in Iranian women. Methods: About 117 women with history of PE and 103 healthy women with a pregnancy not complicated by PE were selected. The genomic DNA was extracted from peripheral blood. Single-nucleotide polymorphisms were genotyped using Real-Time PCR. Results: There was a significant difference between MTHFR c.677C > T polymorphism with PE (p = 0.045). The frequency of C/T heterozygous genotypes were (58% vs. 36%) in the case and control groups, respectively. There were no statistically significant differences between other genetic polymorphisms. Conclusions: The results indicated that the MTHFR c.677C > T polymorphism may be associated with development of PE in Iranian women.

Keywords:

• preeclampsia
• polymorphism
• MTRR c.66A > G
• MTHFR c.677C > T
• MTHFR c.1298A > C
• MTR c.2756A > G

Acknowledgments

Thank you to the all participants in this study. In addition, we would like to thank of Non-communicable diseases research center and Hakim medical genetics center in Neyshabur University. The study was conducted in concordance to the ethical standards of the institution. Thesis code: 203

Disclosure statement

The authors declare that they have no conflict of interests.

Additional information

Funding

This study was supported by a grant from Neyshabur University of Medical Sciences (Grant number IR.NUMS.REC.1396).