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Abstract
Background
With the development of liquid biopsy technology, the demand for noninvasive prenatal testing (NIPT) is increasing rapidly. The aim of the study is to evaluate the effects of different blood collection tubes on plasma cfDNA and NIPT quality control.
Methods
We investigated hemolysis, cfDNA concentration, and fragment distribution within blood samples stored in EDTA, ImproGene, and Streck tubes. The effects of ImproGene and Streck tubes on NIPT quality control were evaluated.
Results
The ImproGene tubes prevented the time-dependent increase of cfDNA concentration and preserved the cfDNA fragment size distribution. For NIPT quality control, there is no significant difference in cfDNA, library concentration, and fetal fraction between ImproGene and Streck tubes samples. GC content of the samples in ImproGene tubes was closer to the human genome.
Conclusion
The ImproGene cfDNA tube has excellent performance and is an effective choice for storing blood samples for NIPT testing or other cfDNA analysis.
Acknowledgments
The authors would like to thank Professor Jiancheng Tu (Department of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University) for helping improve the article.
Disclosure statement
The authors declared that they have no conflicts of interest to this work.
Funding
The author(s) reported there is no funding associated with the work featured in this article.