Abstract
Gold nanoparticles (GNPs) production by the green chemistry approach was examined using a marine-derived fungus Rhizopus oryzae. The test fungi revealed production of gold nanoparticles both, extra and intracellularly. Extracellular biosynthesis was found at the gold chloride concentration 0.25 mM, as evidenced by lavender-purple color of the solution. At other concentrations, intense coloration in biomass was observed indicating intracellular biosynthesis of gold nanoparticles. To the best of the author's knowledge, this is first ever report on extracellular biosynthesis of GNPs by R. oryzae under static condition.