Cytotoxicity and Antimicrobial Studies of Silver Nanoparticles Synthesized Using Psidium guajava L. Extract

Abstract

Polyphenols in guava extract act as reducing as well as stabilizing agent to produce silver nanoparticles. UV-Visible spectra at 420 nm confirmed the formation of silver nanoparticles. The Fourier transform infrared spectroscopy analysis was carried out to identify the functional groups responsible for the bio-reduction of silver. The morphology of silver nanoparticles was confirmed by high-resolution transmission electron microscopy. Ag nanoparticles showed excellent antibacterial activity against Staphylococcus mutans, Bacillus cereus, Eschericia coli, Staphylococcus aureus, and Staphylococcus typhii. In our study, cervical carcinoma HeLa cell line was used to observe the efficacy of cancer cell killing ability of silver nanoparticles.

Keywords:

• silver nanoparticles
• Psidium guajava L.
• cytotoxicity
• antimicrobial activity
• polyphenols
• HRTEM

Acknowledgment

The authors gratefully acknowledge National Centre for Nanoscience and Nanotechnology, University of Madras for FESEM, HRTEM, and EDAX analyses.