Synthesis, characterization, and DNA binding studies of the hematoxylin-Zn(II) complex

ABSTRACT

The binding mechanism of Hematoxylin-Zn(II) complex with Herring sperm DNA has been investigated using UV–vis and fluorescence spectroscopies and viscosity measurement. With the increase in the concentration of complex, the viscosity of DNA decreased, and the binding mode is more detailed: there is partially intercalate binding. Furthermore, besides the intercalate binding, the existence of groove binding was confirmed through the ionic strength experiment in Scatchard analysis. Thermodynamic studies provided some thermodynamic parameters: Δ_rHm^Θ = 1.43 × 10⁵ J mol⁻¹, Δ_rSm^Θ = 576.14 J mol⁻¹, Δ_rGm^Θ_303K = −3.16 × 10⁴ J mol⁻¹, they illustrated that the binding mechanism between HE-Zn(II) complex and hsDNA was driven mainly by entropy.

KEYWORDS:

• Hematoxylin-Zn(II) complex
• herring sperm DNA
• binding mechanism
• acridine orange

Acknowledgment

The authors are grateful for the apparatus support of the Analytical and Testing Center of Sichuan Normal University.

Funding

This work was supported by the National Natural Science Foundation of China under Grant No. 30572254 and Key Fund Project of Sichuan Provincial Department of Education (NO. 14ZA0027).