Ethanol production from acorn starch by tannin tolerance mutant Pachysolen tannophilus

ZHANG NingInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, China

JIANG Jian-ChunInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, ChinaCorrespondence[email protected]

YANG JingInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, China

WEI MinInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, China

ZHAO JianInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, China

XU HaoInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, China

XIE Jing-CongInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, China

TONG Ya-JuanInstitute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu Province, China;National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, Jiangsu Province, China;Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration of the People's Republic of China (SAF), Nanjing, Jiangsu Province, China;Key Laboratory of Biomass Energy and Material, Nanjing, Jiangsu Province, China

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ABSTRACT

The acorn starch physicochemical properties were studied, and the acorn flour was used to ferment fuel ethanol by tannin tolerance mutants. The results showed that the gelatinization temperature of acorn starch lower than cassava starch and corn starch, which indicated that the gelatinization of acorn starch is easier than corn starch and cassava starch; Two tannin-tolerance mutant strains P05 and P18 were obtained after UV irradiation and tannin gradient domestication. Use of mutant P18 may decrease the extraction times of tannin from 5 to 3 times, which simplifies the production process and reduces the ethanol production cost.

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Funding

Foundation from Basic scientific research project of jiangsu province biomass energy and materials laboratory (JSBEM-S-2017011)

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Ethanol production from acorn starch by tannin tolerance mutant Pachysolen tannophilus

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Ethanol production from acorn starch by tannin tolerance mutant Pachysolen tannophilus

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