Saccharomyces cerevisiae and newly isolated Candida boidinii co-fermentation of industrial tea waste for improved bioethanol production

ABSTRACT

Although bioethanol is a suitable alternative to fossil-based fuels, limited technologies or lack of microorganisms with a broad substrate spectrum are serious bottlenecks for lignocellulosic ethanol production. In this context, the current study aimed to optimize bioethanol production from industrial tea waste by applying co-fermentation strategy for the first time. For this purpose, newly isolated xylose fermenter Candida boidinii and Saccharomyces cerevisiae produced 12.1 g/L and 14.1 g/L bioethanol, respectively, when the yeasts were cultivated alone in 20% (w/v) dilute acid pretreated and enzymatically hydrolyzed industrial tea waste. Bioethanol production increased to 21.9 g/L, and 70% improvement was achieved when the co-fermentation strategy was applied under the same conditions. This study shows that co-fermentation of industrial tea waste is an economically viable choice for value-added bioethanol production.

KEYWORDS:

• Candida boidinii
• saccharomyces cerevisiae
• industrial tea waste
• bioethanol
• co-fermentation

Acknowledgments

This study was supported by Ankara University Research Foundation. Project No: 21L0430012.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Ankara University Research Foundation [21L0430012].