A Dorsal Model for Cutaneous Vesicant Injury by 2-Chloroethyl Ethyl Sulfide Using C57BL/6 Mice

Myriane A. Isidore Department of Biochemical Pharmacology/Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA

Michael P. Castagna Department of Biochemical Pharmacology/Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA

Keith E. Steele Divison of Pathology, Department of Immunohistochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA

Richard K. Gordon Department of Biochemical Pharmacology/Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA

Madhusoodana P. Nambiar Department of Biochemical Pharmacology/Division of Biochemistry, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA; Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA

Abstract

To evaluate stem cell-derived therapeutics for cutaneous vesicant injuries, we developed a dorsal exposure model using C57BL/6 black mice and half-mustard, 2-chloroethyl ethyl sulfide (CEES). The dorsal side of a mouse was exposed to 1–5 μl of CEES for 10 minutes and then decontaminated. The data demonstrate that 3 μl of CEES induced edema and erythema that peaked 24 h post exposure. Histopathology showed a central area of deep injury characterized by severe necrosis of epidermis and dermis. The C57BL/6 is a unique model that can be used to unravel the molecular mechanism of injury, identify the effects of black skin pigmentation, and evaluate the efficacy of stem cell therapeutics for cutaneous vesicant exposure.

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A Dorsal Model for Cutaneous Vesicant Injury by 2-Chloroethyl Ethyl Sulfide Using C57BL/6 Mice

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A Dorsal Model for Cutaneous Vesicant Injury by 2-Chloroethyl Ethyl Sulfide Using C57BL/6 Mice

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