The effect of levocabastine hydrochloride on human Tenon’s capsule fibroblasts: Inhibition of proliferation, suppression of DNA synthesis and induction of apoptosis

Abstract

Purpose: To investigate the effects of levocabastine hydrochloride on the profileration, Deoxyribonucleic acid (DNA) synthesis and induction of apoptosis, in human Tenon’s capsule fibroblasts. Methods: The maximum intoxicate concentration was determined by measuring the release of lactate dehydrogenase. Proliferation of fibroblasts was analyzed with a WST-1 method. DNA synthesis was examined by quantifying the incorporation of 5-bromo-2’-deoxyuridine into dividing cells, and apoptosis was analyzed using an Annexin-V-FLUOS Staining Kit. Results: Levocabastine hydrochloride (10⁻⁴ M), ketotifen fumarate (3 × 10⁻⁵ M) and tranilast (10⁻⁴ M) significantly inhibited proliferation of fibroblasts. Levocabastine hydrochloride (10⁻⁴ M), ketotifen fumarate (10⁻⁴ M) and tranilast (10⁻³ M) inhibited DNA synthesis and induced apoptosis. Conclusion: Levocabastine hydrochloride inhibited the proliferation of fibroblasts.

Keywords::

• Levocabastine hydrochloride
• human Tenon’s capsule fibroblast
• DNA synthesis
• apoptosis
• proliferation

Acknowledgments

Declaration of interest: The authors report no conflicts of interest.