Linear mimotope analysis of Iranian cobra (Naja oxiana) snake venom using peptide displayed phage library

Abstract

Snake venom pharmacologically comprises active components. This study aimed to identify mimotopes of Naja oxiana. Phage display peptide library, Ph.D.C7C, was utilized and biopanning carried out on immobilized horse antibodies. Three rounds of selection were performed on purified antibodies. Single phages randomly selected from the third round of biopanning were amplified and subjected to DNA sequencing. DNA sequencing results revealed 35 peptides with unique sequences. Obtained peptides represent some linear mimicking epitopes of venom antigens that involve in interaction with antibodies and could be used for further immunological and venomics study.

Keywords:

• Phage display
• peptide library
• epitope mapping
• snake venom
• venomics

Ethical approval

All experiments were approved by ethical committee of animal care of Pasteur institute of Iran, Tehran, Iran (No. IR.PII.REC.1394.42).

Acknowledgements

We acknowledge with deep respect from Pasteur Institute of Iran, Tehran, Iran for supporting this study.

Disclosure statement

The authors declare that there is no conflict of interest.