A ¹³C-NMR study of exudation and storage of carbohydrates and amino acids in the ectomycorrhizal edible mushroom Cantharellus cibarius

Abstract

¹³C-NMR analyses of Cantharellus cibarius growth media were performed. We found exudation of trehalose and mannitol, which may explain the phenomenon of reproducing Pseudomonas bacteria observed inside fruit bodies. Exudation varied with strain and environment. NMR analyses of stored ¹³C was also performed. Trehalose, mannitol, and arginine were revealed. The mannitol pathway seems to play an important role for trehalose production in this species. This is the first study of the fate of the photosynthetically derived carbon in the highly appreciated edible ectomycorrhizal mushroom Cantharellus cibarius.

• arginine
• chanterelle
• mannitol
• metabolism
• mycorrhiza
• trehalose

We would like to thank Dr. Francis Martin (Equipe de Microbiologie Forestière, INRA-Nancy) for his kind assistance in the ¹³C-NMR analyses of one of the strains. The project had support from the Swedish Council for Forestry and Agricultural Research, The Trygger Foundation and The National University of Mexico (UNAM/DGAPA).