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Abstract
A new species of the Ophiostoma piceae -complex was isolated from bark beetles and the insect galleries of Larix kaempferi in Japan. This species was described as Ophiostoma breviusculum. The fungus was morphologically similar to O. piceae and O. quercus. However the average length of the perithecial necks and synnemata were shorter than for O. piceae and O. quercus. Synnemata morphological characteristics also differentiated O. breviusculum from the other species of the Ophiostoma piceae - complex isolated from conifers. Mating tests demonstrated that this fungus did not produce perithecia with O. floccosum, O. piceae and O. quercus. In phylogenetic trees using rDNA ITS O. breviusculum was placed in a clade with O. canum, O. piceae and O. subalpinum, but when using β-tubulin it was placed into a separate clade.
We are grateful to Futarasan Shinto Shrine in Nikko and the Experimental Forests in Yatsugatake, Agricultural and Forestry Research Center, University of Tsukuba, for providing the samples. Part of the studies was supported by the Global Environment Research Fund, Ministry of the Environment, Japan, to G. Saito and T. Hakamata. Contribution No. 195, Laboratories of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of Tsukuba.
