Three new Lasiodiplodia spp. from the tropics, recognized based on DNA sequence comparisons and morphology

Abstract

Botryosphaeria rhodina (anamorph Lasiodiplodia theobromae) is a common endophyte and opportunistic pathogen on more than 500 tree species in the tropics and subtropics. During routine disease surveys of plantations in Australia and Venezuela several isolates differing from L. theobromae were identified and subsequently characterized based upon morphology and ITS and EF1-α nucleotide sequences. These isolates grouped into three strongly supported clades related to but different from the known taxa, B. rhodina and L. gonubiensis, These have been described here as three new species L. venezuelensis sp. nov., L. crassispora sp. nov. and L. rubropurpurea sp. nov. The three could be distinguished easily from each other and the two described species of Lasiodiplodia, thus confirming phylogenetic separations. Furthermore all five Lasiodiplodia spp. now recognized separated from Diplodia spp. and Dothiorella spp. with 100% bootstrap support.

• Botryosphaeria
• Diplodia
• Dothiorella
• Fusicoccum
• ITS
• molecular phylogenetics
• translation elongation factor EF1-α

We thank Hugh Glen for Latin descriptions and Dianne White for technical support. This work was financed in part by the Australian Research Council DP0343600.