ABSTRACT
Prenatal maternal smoking is associated with low birthweight, neurological disorders, and asthma in exposed children. DNA methylation signatures can function as biomarkers of prenatal smoke exposure. However, the robustness of DNA methylation signatures across child ages, genetic ancestry groups, or tissues is not clear. Using coefficients from a meta-analysis of prenatal smoke exposure and DNA methylation in newborn cord blood, we created polymethylation scores of saliva DNA methylation from children at ages 9 and 15 in the Fragile Families and Child Wellbeing study. In the full sample at age 9 (n = 753), prenatal smoke exposure was associated with a 0.51 (95%CI: 0.35, 0.66) standard deviation higher polymethylation score. The direction and magnitude of the association was consistent in European and African genetic ancestry samples. In the full sample at age 15 (n = 747), prenatal smoke exposure was associated with a 0.48 (95%CI: 0.32, 0.63) standard deviation higher polymethylation score, and the association was attenuated among the European and Admixed–Latin genetic ancestry samples. The polymethylation score classified prenatal smoke exposure accurately (AUC age 9 = 0.77, age 15 = 0.76). Including the polymethylation score increased the AUC of base model covariates by 5 (95% CI: (2.1, 7.2)) percentage points, while including a single candidate site in the AHRR gene did not (P-value = 0.19). Polymethylation scores for prenatal smoking were portable across genetic ancestries and more accurate than an individual DNA methylation site. Polymethylation scores from saliva samples could serve as robust and practical biomarkers of prenatal smoke exposure.
Ethics approval and consent to participate
Participants provided written informed consent for the study. The data used in this manuscript were prepared by the Fragile Families and Childhood Wellbeing Study administrators following approval of the manuscript proposal. These secondary data analyses were approved by the University of Michigan Institutional Review Board (IRB, HUM00129826).
Data availability statement
Code to perform all analyses is available (www.github.com/bakulskilab). Survey data for the Fragile Families and Child Wellbeing study are publicly available (https://fragilefamilies.princeton.edu/documentation). DNA methylation and genetic data in the Fragile Families and Child Wellbeing study are available through the Restricted Use Contract (https://fragilefamilies.princeton.edu/restricted), upon demonstrated Institutional Review Board approval of the proposed research and data protection plan.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Authors’ contributions
FB performed the analysis with contributions from JD, JF, and EBW. FB and KB wrote the paper with all authors contributing to revisions. CM, DN and LS contributed to the design and processing of the DNA methylation data subcohort. DN, CM, KM, EBW, and FB all contributed to the design and conception of the analytic plan. All authors reviewed and revised the manuscript.
Supplementary material
Supplemental data for this article can be accessed online at https://doi.org/10.1080/15592294.2022.2112815