Synthesis of water-soluble fluorescent polymeric glycoconjugate for the detection of cholera toxin

ABSTRACT

Considering inherence optical properties of adjoint polyfluorenes and special functions of water-soluble conjugated glycopolymers, a triazole chain glycoconjugate via one-pot method were rapidly synthesized to prepare a lactate ligand polyfluorene with a clear fluorescent label by a nickel-catalyzed Yamamoto coupling polymerization. The water solubility and biocompatibility of the glycoconjugated polymer were ameliorated when the lactose group introduced as the side chain of the conjugated polymer. As a fluorescent multivalent system of glycoconjugates containing pyranogalactose groups, the interaction between pyranogalactose group and cholera toxin B subunit was studied by fluorescence spectrophotometric titration. PF-Lac has a broad application prospect in the check of cholera toxin and the study of glycoprotein interaction.

KEYWORDS:

• Cholera toxin
• fluorescence quenching
• lactose
• sensors
• water-soluble conjugated polymer

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed here.

Additional information

Funding

This work was supported by the Science and Technology Cooperation Project of Guizhou Province (Grant QKHLHZ-2015–7564) and the PhD Start-up Foundation of Zunyi Medical College (Zhuhai Compus) (Grant F-710)