Immunoglobulin D (IgD) and IgD receptor expression in diffuse large B-cell lymphoma

ABSTRACT

Objective: Immunoglobulin D (IgD) levels are often elevated in patients with autoimmune diseases. However, the oncogenic activities of IgD and IgD receptor (IgDR) in diffuse large B-cell lymphoma (DLBCL) have not been reported in detail. Therefore, we aimed to investigate the expression of IgD and IgDR in patients with DLBCL.

Methods: Membrane IgD (mIgD) and IgDR expression in tissue samples was analyzed using IHC, mIgD and IgDR expression on peripheral blood mononuclear cells (PBMCs) was analyzed by FCM, and secreted IgD (sIgD) level was analyzed by ELISA. Fisher’s exact test and Spearman correlation analysis were used to evaluate the relationship between IgD, IgDR, and clinical parameters.

Results: The pathological lymph nodes of 34 patients with DLBCL were studied, and mIgD and IgDR expression was found in 16 and 19 patients. mIgD and IgDR expression was upregulated in patients with DLBCL and mIgD expression was significantly associated with IgDR expression. Further correlation analysis showed that mIgD expression was correlated with serum β₂-MG level and Hans algorithm as germinal center B (GCB), whereas IgDR expression correlated with serum LDH level, IPI score and GCB. ELISA showed that sIgD level was significantly increased in DLBCL patients and it correlated with serum β₂-MG and LDH levels. FCM showed that mIgD and IgDR expression in PBMCs of patients with DLBCL was significantly higher than that in healthy controls.

Conclusion: Our findings suggest that overexpression of IgD and IgDR is an abnormal activation state in DLBCL.

KEYWORDS:

• Immunoglobulin D
• IgD receptor
• diffuse large B-cell lymphoma
• β₂-microglobulin
• serum LDH
• International lymphoma prognostic Index score
• germinal center B
• peripheral blood mononuclear cells

Acknowledgments

The authors acknowledge Doctor Da-Bin Huang and Doctor Jie Chen in Affiliated Provincial Hospital of Anhui Medical University, for their help in pathological specimens and blood samples collecting.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research was supported by the National Natural Science Foundation of China [grant numbers 81330081; 81673444; 81603121], Grants for Scientific Research of BSKY [grant numbers XJ201629 and XJ201630] from Anhui Medical University.