Analysis of gene mutation characteristics in patients with chronic neutrophilic leukaemia

ABSTRACT

Objective: This study aims to investigate the gene mutation characteristics of chronic neutrophilic leukaemia (CNL).

Method: This study retrospectively analyses the molecular biological characteristics, laboratory characteristics and clinical data of four patients with CNL that were admitted in the second Hospital of Shanxi Medical University from May 2014 to October 2016. On the basis of the molecular biological data of 22 patients with CNL and 4 patients with CNL, we further analysed the characteristics of CNL molecular mutation.

Results: Two out of the four patients with CNL were carriers of colony-stimulating factor 3 receptor (CSF3R) mutation, among which two were carriers of CSF3R T618I mutation combined with ASXL1 mutation and SETBP1 mutation, and two were only carriers of JAK2 V617F mutation. According to the molecular biological data of 22 patients with CNL, 20 patients were positive for CSF3R mutation. Two patients were positive for JAK2 V617F mutation. A total of 10 patients were positive for SETBP1 mutation which was correlated with the CSF3R T618I gene mutation (P = 0.03). A total of 13 patients were positive for ASXL1 mutation. No patients carried mutations in ASXL2 and MPL genes.

Conclusion and Discussion: CSF3R mutation is the main tumorigenic mutation in CNL, in which CSF3R T618I mutation is the main mutation, and an extremely small number of CNL patients may be caused by JAK2 V617F mutation. SETBP1 and ASXL1 are the most common concomitant mutations in CNL with CSF3R mutation, and SETBP1 and CSF3R T618Imutations may have a certain correlation.

KEYWORDS:

• Chronic neutrophilic leukaemia
• mutation
• CSF3R
• JAK2 V617F
• SETBP1
• ASXL1
• CALR
• Myeloproliferative neoplasm

Acknowledgements

This work was supported by National Natural Science Foundation of China (NO.81670126; NO.81500104), The Shanxi Natural Science Foundation of China (NO.201801D111003;NO.201801D221409).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by National Natural Science Foundation of China: [Grant Number 81670126; 81500104]; The Shanxi Natural Science Foundation of China: [Grant Number 201801D111003; 201801D221409].