Bacterial Community Evaluation During Establishment of Tall Fescue (Festuca arundinacea) in Soil Contaminated with Pyrene

ABSTRACT

A greenhouse study was conducted to monitor microbial community dynamics related to contaminant concentration and plant growth during the phytoremediation of pyrene. Soil microbial communities in both bulk and rhizosphere soils were assessed using a polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE). However, no correlation between pyrene concentration and bacterial community shifts was observed. Even though plants significantly enhanced pyrene degradation (undetectable after 91 d for planted treatments compared with 82 mg/kg for unplanted treatments after 147 d with initial concentration of 758 mg/kg), biodegradation may have been accomplished by the pre-existing microbial community. It is also possible that the method was unable to pick up subtle community shifts, considering that the carbon source from pyrene was only 3.7% of the existing soil organic matter. This research suggests that plants significantly enhance degradation and mineralization of pyrene in soil, although there was no conclusive evidence supporting any specific bacterial activity responsible for this enhancement.

ACKNOWLEDGEMENTS

We would like to thank Dr. Cindy Nakatsu for providing laboratory facilities in which to conduct this research. We also thank Judy Lindell, Corinne Ackerman, and Robert Kim for experimental assistance. This research was supported by the U.S. Environmental Protection Agency.