Abstract
The purpose of this study was to explore the mRNA expression of Na, K-ATPase alpha subunit in Litopenaeus vannamei (Boone, 1931) in response to salinity stress. L. vannamei acclimated to 30 ppt, was transferred to 30 (control), 15, 7.5 and 1 ppt for 0.5, 1, 3, 6, 12 and 24 h. Partial Na, K-ATPase alpha subunit expression in both gills and hepatopancreas was examined using quantitative real-time PCR, showing that there was no significant difference within 3 h after treatment (P>0.05). However, there was a suddenly increase to a peak value at 6 h (P<0.05), then the expression level decreased from 12 h to 24 h. Similar results were obtained for the activities of the Na, K-ATPase. These results suggest that L. vannamei Na, K-ATPase expression is stimulated by salinity stress and that it may play important roles in regulation of the ion density in vivo. To our knowledge, this is the first report on the mRNA expression of Na, K-ATPase alpha subunit in L. vannamei in response to salinity stress.
Published in collaboration with the University of Bergen and the Institute of Marine Research, Norway, and the Marine Biological Laboratory, University of Copenhagen, Denmark
Published in collaboration with the University of Bergen and the Institute of Marine Research, Norway, and the Marine Biological Laboratory, University of Copenhagen, Denmark
Acknowledgements
Project support was provided in parts by grants from the National 863 Program (grant No. 2006AA10A406&2010AA10A401); the National Key Technologies R&D program (grant No. 2006BAD01A13-03); Chinese Academy of Sciences Program (grant No. KZCX2-YW-Q07-03 and KSCX2-YW-N-089) and the Science and Technology Program of Guangdong Province (grant No. A200899A02 & A200901B03 and 2009B091300088) to CQH (LPZ), and from LMB of the South China Sea Institute of Oceanology (grant No. 50601-43) and the 48th China Postdoctoral Science Foundation to HYS.
Notes
Published in collaboration with the University of Bergen and the Institute of Marine Research, Norway, and the Marine Biological Laboratory, University of Copenhagen, Denmark