Effects of temperature and irradiance on the growth of regenerated juveniles from Sargassum fusiforme holdfasts

ABSTRACT

To improve the quality of Sargassum fusiforme seedlings, suitable strains of juveniles regenerated from holdfasts need to be bred. In this study, effects of different temperatures (16, 20, and 24 °C) and irradiances (100, 120, and 160 µmol photons·m⁻²·s⁻¹) on the growth of juveniles (new individuals) from the holdfasts of S. fusiforme were investigated to provide strategies for the cultivation of vegetative propagation seedlings of S. fusiforme. The results demonstrated that during cultivation at 24 °C, the regenerated juveniles rotted after twenty-one days, and their relative growth rate, photosynthetic activity and pigment content decreased substantially. The low temperature of 16 °C also decreased their photosynthetic activity and growth considerably. At a high irradiance of 160 µmol photons·m⁻²·s⁻¹, the growth rate and photosynthetic activity decreased. Under conditions of 20 °C and 120 µmol photons·m⁻²·s⁻¹, juveniles showed higher photosynthetic activity and nitrate uptake rates, which increased the growth of the juveniles and lateral branches and promoted the accumulation of photosynthetic pigment. Therefore, culture conditions of 20 °C and 120 µmol photons·m⁻²·s⁻¹ can be applied for early indoor cultivation of regenerated juveniles of S. fusiforme. This study provided technical support for solving the problem of single provenance of S. fusiforme seedlings.

KEYWORDS:

• Sargassum fusiforme
• seedling breeding
• temperature
• irradiance
• holdfast
• photosynthesis

Acknowledgements

The authors would like to acknowledge the participating staff of the project for their helpful assistance during this research. The project was funded by the Wenzhou Science and Technology Plan Project (No. N20220005), the National Key Research and Development Program of China (No. 2018YFD0901500), the National Natural Science Foundation of China (No. 41706147 and 41876124).

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No potential conflict of interest was reported by the author(s).

Correction Statement

This article has been corrected with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by National Key Research and Development Program of China: [Grant Number 2018YFD0901500]; National Natural Science Foundation of China: [Grant Number 41706147]; Wenzhou Science and Technology Plan Project: [Grant Number N20220005].