Fabrication of a nano-biocatalyst for regioselective acylation of arbutin

ABSTRACT

A nano-biocatalyst has been successfully prepared via coordination between zinc ion and the nitrogen atoms of the amide groups in the Candida sp. 99-125 lipase. The as-prepared nanoparticle has an excellent hierarchical structure with a large number of separate petals. In regioselective acylation of arbutin, the nano-biocatalyst showed enhanced enzyme activities to about 4.3-folds than the free enzyme. Meanwhile, the prepared nano-biocatalyst exhibited good operational reusability and only about 6% of enzyme activity has lost during 10 continuous reaction-recycles.

GRAPHICAL ABSTRACT

KEYWORDS:

• Enzyme immobilization
• nano-biocatalyst
• hybrid nanoparticle
• regioselective transesterification
• arbutin

Disclosure statement

No potential conflict of interest was reported by the authors.

Notes on contributors

Lu Zheng is a doctoral candidate in Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences (Jilin University, Changchun, China). Her research has focused on the biocatalysis and biotransformation.

Xiaona Xie is working in the first hospital of Jilin University in Changchun City. Now, she is studding in Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education (Jilin University, Changchun, China) for the synthesis and development of chiral drugs.

Zhi Wang was born in 1973. He obtained his Ph.D. degree in 2003. Recently, his research has focused on the enzymatic resolution of chiral compounds, green chemistry and medicinal chemistry.

Yuxin Zhang is studding as a doctoral candidate in School of Life Sciences, Jilin University. Her research has focused on the biocatalysis and biotransformation.

Lei Wang is working in Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education (Jilin University, Changchun, China) as a professor. Recently, his research has focused on the new synthetic methodologies to synthesize bioactive compounds.

Xiuyun Cui is studding as a master candidate in School of Life Sciences, Jilin University. Her research has focused on the synthesis and development of new drugs.

He Huang is studding as a master candidate in School of Life Sciences, Jilin University. Her research has focused on the synthesis of bioactive compounds.

Hong Zhuang, female, born in 1974, professor. She is working in College of Food Science and Engineering, Jilin University, Changchun. Her research interest is food chemistry, mainly engaged in the design, development and detection of functional foods.

Additional information

Funding

This work was supported by Fund of Basic Scientific Research from Jilin University (for Constructing the Scientific and Technological Platform, 2017) [grant number 2017]; the Foundation of Changchun BC&HC Pharmaceutical Technology Co., Ltd [grant number 2017220002000304].