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ABSTRACT
Sago processing spent waste disposal causes severe impact on agriculture soil surface water, and air. Bio-coversion of sago waste to fuel would be eco-friendly approach and recover the energy remaining in the spent waste. The present research foucses on the production of butanol using Enterococcus hirae novel strain shown resistant to 4% of butanol. Butanol production capacity of isolate was found to be 5.70+0.06g/L and this was increased to 6.95+g/L when it was optimized with coconut oil cake (13%), sago effluent (40%), glucose, tryptone, 37 C and pH 7.0. The results are promosing and sago spent waste can be used as cheaper subtrate for the sustaible produciton of bio-butanol.
Acknowledgements
The authors are thankful to the Vice-Chancellor and Registrar of Periyar University, Salem, and CUSAT, Kochi, for providing facilities to conduct research work. We also thank Dr. Nasib Qureshi, USDA, ARS, and Dr. John Ravindar, PDF, Hong Kong, for their valuable and timely help. We also thank DST-FIST (Ref No. SR/FST/LSI-640/2015(C) Dt. 30/5/2016) for the instrumentation facilities.