Effects of a combination of phytase and multi-carbohydrase enzymes in low-density corn–soybean meal based diets on growth performance and ileal nutrients digestibility of male broilers

Abstract

A study was conducted to evaluate the effect of dietary supplementation of phytase and carbohydrase on growth performance and ileal digestibility of nutrients in broilers fed low-density corn–soybean meal-based diets. A total of 280 one-day-old Ross 308 male broilers were randomly assigned to 7 dietary treatments, with 4 replicates of 10 birds. Dietary treatments were (1) full nutrient specification without enzyme supplementation (positive control; PC), (2) diet with reduced nutrients as a phytase matrix (negative control; NC), (3) NC + phytase (500 FTU/kg diet), 4) NC + multi-carbohydrase (0.1 g/kg), (5) NC + phytase + multi-carbohydrase, (6) diet with reduced nutrients as phytase and multi-carbohydrase matrices; low negative control (LNC), and (7) LNC + phytase + multi-carbohydrase. Broilers fed either NC or LNC diets had lower body weight gain, the European production efficiency factor, and a higher feed conversion ratio than those fed PC diet (p < .01). Broilers fed LNC diet had lower dry matter (DM) and crude protein (CP) digestibility, and apparent ileal digestible energy (AIDE) than those fed PC diet (p < .01). Multi-carbohydrase supplementation on top of phytase-containing NC diets resulted in a similar effect on performance and digestibility of DM and AIDE in broilers compared with LNC diets containing phytase and multi-carbohydrase. Digestibility of CP was lower in NC + multi-carbohydrase groups than NC + phytase + multi-carbohydrase (p < .01). In conclusion, multi-carbohydrase was successful in improving broiler performance and nutrients digestibility in the diet based on the corn–soybean meal with lower nutrient density; thus, when supplementing it as a second enzyme in diets containing phytase, its matrix value should be considered for least-cost feed formulation.

HIGHLIGHTS

• Phytase and carbohydrase matrix values should be considered when enzyme combination using in C-SBM diet.

• Carbohydrase improved broiler performance and nutrients digestibility in C-SBM diet with lower nutrient density.

• Cost-effectiveness use of enzyme combination in diets could reduce nutrient excretion.

Keywords:

• Broiler
• multi-carbohydrase
• phytase
• growth performance
• nutrient digestibility

Ethical Approval

All experimental procedures were conducted according to the international protocols and approved by Research Committe of University of Guilan, IRAN.

Acknowledgements

The authors would like to appreciate Research Council of University of Guilan and EASY BIO Inc. for the support.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was financially supported by EASY BIO, Inc., Korea.