Hepatic acute-phase response, antioxidant biomarkers and DNA fragmentation of two rabbit breeds subjected to acute heat stress

Abstract

Acute heat stress (AHS) is known to affect rabbit production and well-being negatively. Yet, early acute heat stress effect on later physiological response is poorly studied. Our study aimed to provide more knowledge of rabbit physiological response to early acute heat stress in two distinguished rabbit breeds. A total of 120 males of New Zealand White (NZW) and Baladi Black (BB) rabbits five weeks old were recruited and randomly divided into two sub-groups, control groups (Control) reared in a controlled environmental chamber 28 °C, and 40% RH and early acute heat stress groups (HS) exposed to 36 °C and 62% RH for 6 hours. At 13 weeks, rabbits of all groups were subjected to the same AHS conditions. Our results, irrespective of breeding differences, demonstrate a significant (p < .05) increase in inflammation markers (TNFα, IL-6 and lipid peroxidation), acute-phase proteins (ceruloplasmin, α-1-acid glycoprotein), and DNA fragmentation with a significant reduction in liver antioxidant enzymes concentrations (glutathione reduced, superoxide dismutase, and catalase) in HS groups compared to breed relative control groups. Furthermore, when all experimental groups at 13 weeks of age were subjected to the same stress conditions, the early HS group showed the same significant adverse physiological effects. It can be suggested that rabbits from different genetic groups are vulnerable to acute heat stress exposure at the early stage of life compared to later stages.

Highlights

• Heat stress is one of the most critical issues facing rabbit production.

• Exposure young rabbits to acute heat stress had adverse effects on physiological parameters in later life.

• Rabbits exposed to acute heat stress at an early age did not produce thermo-tolerance ability to later time heat exposure.

Keywords:

• Acute heat stress
• acute-phase protein
• DNA fragmentation
• antioxidant status
• rabbits

Acknowledgments

This paper is based upon work supported by the National Research Center (#12050406).

Animal welfare statement

The authors confirm that they have followed EU standards for the protection of animals used for scientific purposes.

Ethical approval

The experimental design and all the research protocols were approved by the Medical Research Ethics Committee (MREC) of the National Research Centre with ethical approval code 20/128.

Disclosure statement

The authors reported no potential conflict of interest.

Additional information

Funding

This research was supported by the National Research Centre [#12050406].