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Abstract
Wolfberry fruit wine (WFW) is widely used as a global functional food to improve the immune system and prevent human disease. A total of 36 bottled WFWs were randomly collected in China between 2005 and 2010. Samples were analysed for the presence of ochratoxin A (OTA) using immunoaffinity column (IAC) clean-up and high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Positive results were confirmed by liquid chromatography–electrospray ionisation–tandem mass spectrometry (LC-ESI-MS/MS). The limit of detection (LOD), based on a signal-to-noise ratio of 3, was 0.05 ng mL–1. Recoveries ranged from 78.3% to 94.7% and relative standard deviations from 1.1% to 4.3% within the spiking range of 0.2–20 ng mL–1. OTA was detected in one sample, below the maximum allowable limit as established by the European community.
Acknowledgements
This work was supported by Important New Drug Research Project of the Ministry of Science and Technology of China (2009ZX09502-025, 2012ZX09301-002-001) and Scientific Research Project of Traditional Chinese Medicine Vocation in 2008 (200807042) and National Science Foundation of China (No. 81173539). We are grateful to Dr. Guoping Zhou and Ms. Peilin Kuang for technical support.