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ABSTRACT
Aflatoxin B1 (AFB1) occurrence in peanut oil samples randomly collected from family workshops in western Guangdong during 2016–2017 (n = 427) was surveyed. AFB1 content was screened by enzyme-linked immunosorbent assay (ELISA) protocol and analytically confirmed with an UPLC-MS/MS method. The limit of detection of the ELISA method was 1.08 μg kg−1. The recovery values ranged from 84.4 to 92.6% with relative standard deviations of 2.2 to 4.8%. AFB1 was quantified in 47 samples (22.5%) with a range of 15.4–49.9 μg kg−1 in 2016 and in 33 samples (15.1%) with 8.8–22.2 μg kg−1 in 2017, respectively. The AFB1 contamination in peanut oil was season-depended in western Guangdong with the worst case in spring (24.2–37.9%). Overall, a significant reduction of AFB1 occurrence was observed in western Guangdong after 2016. It is advisable to control AFB1 in bulk and self-pressed oil from family workshops and regulate it mandatorily if necessary.
Acknowledgments
We are grateful to Mrs. Lili HE and Mr. Qing AI (R-Biopharm Analysis Systems Trading (Beijing) Co., Ltd.) for the assistance during the validation of analytical methods.
Disclosure statement
No potential conflict of interest was reported by the authors.
Supplementary material
Supplemental data for this article can be accessed here.
