Pyrrolizidine alkaloids in food: downstream contamination in the food chain caused by honey and pollen

Abstract

In recent years, there has been a steadily growing number of published data on pyrrolizidine alkaloids (PAs) in honey and pollen. This raises the question whether honey and/or pollen used as ingredients in food processing might provoke a downstream contamination in the food chain. Here we addressed two different facets in connection with PAs in honey and pollen. First, we analysed the PA content of several food types such as mead (n = 19), candy (n = 10), fennel honey (n = 9), soft drinks (n = 5), power bars and cereals (n = 7), jelly babies (n = 3), baby food (n = 3), supplements (n = 3) and fruit sauce (n = 1) that contained honey as an ingredient in the range of 5% to approximately 37%. Eight out of 60 retail samples were tested as being PA-positive, corresponding to 13%. Positive samples were found in mead, candy and fennel honey, and the average PA content was calculated to be 0.10 µg g^–1 retronecine equivalents (ranging from 0.010 to 0.484 µg g^–1). Furthermore, we investigated the question whether and how PAs from PA pollen are transferred from pollen into honey. We conducted model experiments with floral pollen of Senecio vernalis and PA free honey and tested the influence of the quantity of PA pollen, contact time and a simulated honey filtration on the final PA content of honey. It could clearly be demonstrated that the PA content of honey was directly proportional to the amount of PA pollen in honey and that the transfer of PAs from pollen to honey was a rather quick process. Consequently, PA pollen represents a major source for the observed PA content in honey. On the other hand, a good portion remains in the pollen. This fraction is not detected by the common analytical methods, but will be ingested, and it represents an unknown amount of ‘hidden’ PAs. In addition, the results showed that a technically and legally possible honey filtration (including the removal of all pollen) would not be an option to reduce the PA level of the final product significantly.

Keywords:

• gas chromatography-mass spectrometry (GC/MS)
• clean-up
• natural toxicants
• alkaloids
• honey
• plants
• processed foods
• pyrrolizidine alkaloids

Acknowledgements

This work was supported by the Deutsche Forschungsgemeinschaft (DFG), Bonn, to P.S. (Project Numbers SCHR 211/22-1 and SCHR 211/23-1) and to T.B. (Project Numbers BE 3200/1-1, BE 3200/3-1 and BE 3200/3-2).