Progesterone receptor up-regulation: a diagnostic tool for the illicit use of oestrogens in adult beef cattle

Abstract

The monitoring of gene regulation via mRNA levels to detect anabolic sex steroid administration in cattle is a novel approach to detecting the illicit treatment of livestock in meat production. A previous study revealed that progesterone receptor (PR) gene expression levels were increased in the bulbourethral glands and prostates of 17β-oestradiol-treated prepubertal calves, suggesting that the PR can be used as a specific molecular biomarker for oestrogen treatment. The aim of this study was to verify the specificity and applicability of the PR to detect the illegal use of 17β-oestradiol in sexually mature beef cattle. Accessory sex glands were sampled from 42 male beef cattle that were divided into six experimental groups, including two control groups, K1 and K2. Group A cattle were treated with 17β-oestradiol (five weekly intramuscular doses of 20 mg), and group B cattle were treated with dexamethasone (40 daily doses of 0.7 mg per os). Group C cattle received an implant of Revalor-200 (200 mg of trenbolone acetate and 20 mg of 17β-oestradiol), and group D cattle received Revalor-200 plus dexamethasone (0.7 mg daily per os). 17β-Oestradiol, either alone or in combination with other steroids, up-regulated the PR gene and protein expression, even in the absence of detectable histological changes in the accessory sex glands, confirming the high sensitivity of PR gene expression as an indirect diagnostic screening tool to detect illicit oestrogen treatment in sexually mature male bovine.

Keywords:

• progesterone receptor
• gene expression
• beef cattle
• 17β-oestradiol
• growth promoters

Acknowledgments

This work was partially funded by the Regione Piemonte 2009 project “Prevenzione dell’uso di anabolizzanti in zootecnia: le biotecnologie nello sviluppo di disciplinari per la qualità e salubrità della carne e derivati” and the Ministero delle Politiche Agricole Alimentari and Forestali SAFORISK project “Prevenzione dell’uso di anabolizzanti in zootecnia Creazione di Marchio a difesa degli allevamenti italiani.” We would like to thank COOP ITALIA, Domenico Palmerini, and Alessandra Sereno for the technical support provided and the Reference Center of Comparative Pathology “Bruno Maria Zaini” of the Faculty of Veterinary Medicine.