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Food Additives & Contaminants: Part A Volume 29, 2012 - Issue 8
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Original Articles

Raman spectroscopic determination of norbixin and tartrazine in sugar

Ute Uhlemann Institute of Physical Chemistry and Abbe-Center of Photonics, Friedrich-Schiller-Universität, Jena, Germany
,
Katharina K. Strelau Institute of Physical Chemistry and Abbe-Center of Photonics, Friedrich-Schiller-Universität, Jena, Germany
,
Karina Weber Institute of Physical Chemistry and Abbe-Center of Photonics, Friedrich-Schiller-Universität, Jena, Germany
,
Paulo Augusto Da Costa Filho Analytical Science Quality and Safety Department, Nestlé Research Center, Lausanne, Switzerland
,
Petra Rösch Institute of Physical Chemistry and Abbe-Center of Photonics, Friedrich-Schiller-Universität, Jena, Germany
&
Jürgen Popp Institute of Physical Chemistry and Abbe-Center of Photonics, Friedrich-Schiller-Universität, Jena, Germany; Institute of Photonic Technology, Albert-Einstein-Straße 9, D-07745 Jena, GermanyCorrespondence[email protected]
Pages 1244-1255 | Received 06 Jan 2012, Accepted 03 May 2012, Published online: 29 Jun 2012
 
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Abstract

In this paper, a method for the detection of norbixin and tartrazine in sugar by means of resonance Raman spectroscopy is presented. The extraction was done in four steps using methanol and the measurements were performed in aqueous solution. The excitation wavelength was 514 nm for norbixin and 488 nm for tartrazine samples. The characteristic resonance Raman signals of the dyes were fitted by different functions. Depending on the R 2 values of the different fits, each spectrum was classified as positive or negative response. A detection limit of 250 ng g−1 for norbixin and 989 ng g−1 for tartrazine in solid sugar samples could be reached by logistic regression.

Acknowledgements

We gratefully acknowledge the Free State of Thuringia and the European Union (EFRE) for financial support under support code 2008FE9112 (BioOptiSens). Furthermore, we thank our technical staff Anna Schmidt, Gisela Persch and Petra Weiß for the preparation of the samples.

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