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Original Articles

Classification of stevia sweeteners in soft drinks using liquid chromatography and time-of-flight mass spectrometry

, , , &
Pages 2043-2049 | Received 02 Jul 2013, Accepted 07 Sep 2013, Published online: 29 Oct 2013
 

Abstract

The aim of this study was to develop a comprehensive analytical method for the characterisation of stevia sweeteners in soft drinks. By using LC and time-of-flight MS, we detected 30 steviol glycosides from nine stevia sweeteners. The mass spectral data of these compounds were applied to the analysis to determine steviol glycosides in nine soft drinks. On the basis of chromatographic data and principal-component analysis, these soft drinks were classified into three groups, and the soft drinks of each group, respectively, contained high-rebaudioside A extract, normal stevia extract or alfa-glucosyltransferase–treated stevia extract.

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