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Original Articles

Isolation and identification of novel propoxyphenyl thiosildenafil found in natural health food product

, , , , &
Pages 1-6 | Received 26 Jul 2013, Accepted 12 Oct 2013, Published online: 02 Jan 2014
 

Abstract

A novel phosphodiesterase-5 (PDE-5) inhibitor was found in a natural health food product. The previously unknown sildenafil analogue was isolated using preparative HPLC. The structure of the compound was elucidated using HPLC with diode array detection (DAD), time-of-flight mass spectrometry (TOF/MS), and nuclear magnetic resonance spectroscopy (NMR). An [M + H]+ ion was detected at m/z 505.2077 by LC-TOF/MS that was consistent with C23H32N6O3S2 (–0.98 ppm). By NMR analysis, the analogue was identified as 1-methyl-5-(5-(4-methylpiperazin-1-ylsulfonyl)-2-propoxyphenyl)-3-propyl-1H-pyrazolo[4,3-d]pyrimidine-7(6H)-thione. In this structure, the ethoxy group of thiosildenafil was substituted by a propoxy group of the unknown compound. Therefore, this novel thiosildenafil analogue was named propoxyphenyl thiosildenafil.

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