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Articles

Fast and simple determination of moroxydine residues in pig and chicken samples by ultra-performance liquid chromatography-tandem mass spectrometry

, , , , & ORCID Icon
Pages 2111-2119 | Received 28 Mar 2018, Accepted 04 Aug 2018, Published online: 13 Sep 2018
 

ABSTRACT

A general solid-phase extraction (SPE) method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of moroxydine residues in pig and chicken samples has been developed. After extraction and purification of real samples, moroxydine residues were detected using a hydrophobic interaction liquid chromatography column with an optimised mobile phase composition. The extraction reagents, the kind of SPE columns and the type of eluents were optimised to achieve the maximum extraction efficiency. The matrix effects from the animal tissue influenced the quality of the quantitative data obtained. Under the optimised conditions, the moroxydine residues in pig and chicken samples spiked at three levels (1.0 μg/kg, 5.0 μg/kg and 10.0 μg/kg) were determined with good recoveries (61.5%–105.4%) and adequate relative standard deviations (3.2%–13.0%). In pig and chicken samples, the limit of detection (LOD) was 0.3 μg/kg, and the limit of quantification (LOQ) was 1.0 μg/kg. A sufficiently linear relationship in the range of 1.0 μg/kg–20.0 μg/kg was achieved with a good correlation coefficient (R2 ≥ 0.99).

Graphical Abstract

Acknowledgments

This work was financially supported by the Beijing Natural Science Foundation (2162008) and Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplemental material

Supplemental data for this article can be accessed on the publisher’s website.

Additional information

Funding

This work was supported by the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology ; Beijing Natural Science Foundation [2162008].

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