Tri-octahedral bentonites as potential technological feed additive for Fusarium mycotoxin reduction

ABSTRACT

In 2009 the EU Regulation 386/2009 established a new functional group of feed additives called “substances for reduction of the contamination of feed by mycotoxin”. Later, di-octahedral bentonite (1 m558) was authorised, as an anti-aflatoxin additive, being the only additive of this group authorised to date. This work aims to demonstrate the effectiveness of other bentonites, such as tri-octahedral bentonites, versus Fusarium-mycotoxins, since very few adsorbents have proved their effectiveness in relation to this group of mycotoxins. For this purpose, 7 bentonites (six of them tri-octahedral) and 7 commercial adsorbents, added at 0.02% (w/v), were assayed in an in vitro adsorption experiment using two simulated gastro-intestinal (GI) juices (pH 1.3 and 6.8) versus zearalenone (ZEN: 0.1–5 mg/L), fumonisin B₁ (FB₁: 1–10 mg/L) and deoxynivalenol (DON: 2–10 mg/L). Mycotoxin adsorption data were fitted to Langmuir and Freundlich isotherms. In vitro adsorption experiments showed that ZEN and FB₁ (in the latter case only in acid medium) were partially adsorbed, while the adsorption of DON was negligible. Moreover, the increase of adsorbent dose (up to 0.20%, w/v) significantly improved the in vitro adsorption of ZEN and FB₁, reaching >90% of adsorption. The present work proposes the use of some tri-octahedral bentonites as feed additives for Fusarium-mycotoxin reduction.

KEYWORDS:

• Tri-octahedral bentonite
• Fusarium mycotoxins
• animal feed
• decontamination
• adsorption isotherms

Acknowledgments

The authors are grateful to the following institutions: Spanish government (projects AGL2014-52648-REDT and RTC-2015-3508-2–ERDF. A way to make Europe, funded by the Ministry of Economy and Competitiveness of the Government of Spain and by the European Regional Development Fund of the European Union with the objective of “Promoting technological development, innovation and quality research”) and to XaRTA (The Catalonian Reference Network in Food Technology) for funding. P. Vila-Donat thanks MINECO for her postdoctoral contract.

Declaration of interest statement

The authors declare there are no conflicts of interest.

Additional information

Funding

This work was supported by Spanish government funds (MINECO) MICOBEN project [RTC-2015-3508-2] and by the national network on mycotoxins and toxigenic fungi and their decontamination processes (MICOFOOD) [AGL2014-52648-REDT].