Detoxification of aflatoxin B₁ in broiler chickens by a triple-action feed additive

ABSTRACT

The aim of this study was to evaluate the detoxification of aflatoxin B₁ (AFB₁) in vitro and in broiler chickens using a triple-action compound mycotoxin detoxifier (CMD). Response surface methodology (RSM) was used to evaluate AFB1 detoxification in artificial gastrointestinal fluid (AGIF) in vitro. The AFB₁-degradation rate was 41.5% (P < .05) when using a compound probiotic (CP) in which the visible counts of Bacillus subtilis, Lactobacillus casein, Enterococcus faecalis and Candida utilis were 1.0 × 10⁵, 1.0 × 10⁵, 1.0 × 10⁷ and 1.0 × 10⁵ CFU/mL, respectively. When CP was combined with 0.1% AFB₁-degrading enzyme to give CPADE, the AFB₁-degradation rate was increased to 55.28% (P < .05). The AFB₁-removal rate was further increased to above 90% when CPADE was combined with 0.03% montmorillonite to make CMD. In vivo, a total of 150 one-day-old Ross broilers were allotted to 3 groups, 5 replications for each group, 10 broilers in each replication. Group A: basal diet, Group B: basal diet with 40 μg/kg AFB₁, Group C: basal diet with 40 μg/kg AFB₁ plus CMD. The feeding experiment period was 21 d. The results showed that broiler growth was increased, and AFB₁ residues in serum, excreta and liver were decreased by CMD addition in broiler diet (P < .05). In conclusion, CMD was able to remove AFB₁ efficiently in vitro and to increase broiler production performance and reduce AFB₁ residues in the chickens.

KEYWORDS:

• Aflatoxin B₁
• AFB₁-removal rate
• broilers
• compound mycotoxin detoxifier
• production performance
• tissue residues

Disclosure statement

The authors declare that there is no any conflict of interest.

Additional information

Funding

This research was funded by the Natural Science Foundation of Henan Province, China [182300410029]; Henan Innovation and Demonstration Project, China [201111311100]; Xinxiang Key Scientific and Technological Project, China [ZD19005].