Development of UPLC-MS/MS method for determining hainanmycin in foods of animal origin

Abstract

Hainanmycin is a polyether antibiotic. Toxicological studies have shown the adverse effects of hainanmycin on animals and humans. At present, no study is available on the detection of hainanmycin in edible tissues of animals. Hence, a fast and accurate detection method for hainanmycin is essential. This study aimed to develop a new analytical method based on ultra-high-performance liquid chromatography-tandem mass spectrometry to detect hainanmycin in 10 matrices, including milk, eggs, fat, kidney, muscles and livers of chicken, beef and sheep. The limit of detection and the limit of quantitation of the 10 matrices were 0.1–0.4 μg/kg and 0.25–1 μg/kg, respectively, and were far below the maximum residue limits of other polyether anticoccidial drugs (1–150 μg/kg). The recoveries of hainanmycin ranged from 79% to 105%, and the relative standard deviation ranged from 2.8% to 12.0%. The research results prove that the proposed method is operational and simple in detecting hainanmycin, and has high precision and accuracy in a variety of matrices.

Keywords:

• Food analysis
• hainanmycin
• LC-MS/MS
• LLE
• residue analysis

Author contributions

Ke-xin Chi: Conceptualization, methodology, investigation, writing – original draft, supervision, funding acquisition, and project administration. Yun-fei Xie: Investigation, methodology, and data curation. Hang Yu: Validation. Ya-hui Guo: Validation, and resources. Yu-liang Cheng: Supervision. Wei-rong Yao: Conceptualization, methodology, funding acquisition, and writing – review and editing.

Disclosure statement

The authors have no conflicts of interest concerning the submission of this manuscript. They declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this study.

Additional information

Funding

The work described in this article was supported by the National Natural Science Foundation of China [32172326], the National Key R&D Program of China [2020YFC1606800], the Modern Agriculture in Jiangsu Province – key and general projects [BE2019362], the Science and Technology Planning Project of Jiangsu Market Supervision and Administration Bureau [KJ204132, KJ21125006], the Open Project of Engineering Research Center of Dairy Quality and Safety Control Technology of Ministry of Education of China [R202101], the Suzhou Science and Technology Development Plan [SS2019016], the Project funded by China Postdoctoral Science Foundation [2020M680064] and the Postdoctoral Research Startup Fee of Jiangnan University [1025219032200190].