Determination of extractable and non-extractable florfenicol residues as florfenicol amine in eggs by UPLC-MS/MS

Abstract

Some florfenicol (FF) metabolites have a strong binding affinity towards biomolecules in the edible tissues of some food animals. These bound FF residues cannot be extracted directly from edible tissues with organic solvents and are present in higher concentrations even than solvent extractable residues. In this study, an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to detect the total residues of FF in eggs, by quantifying the metabolite florfenicol amine (FFA). The sample was hydrolyzed at 95–100 °C for 4 hours to release sample-matrix bound residues and convert them all into FFA. The hydrolyzed sample was washed with ethyl acetate to remove interfering substances, extracted with ethyl acetate under alkaline conditions, purified by solid phase extraction and quantified by UPLC-MS/MS. The recoveries of FFA in eggs ranged from 91.2 to 102.4%, with an RSD ≤ 10.9%. The LOD and LOQ were 0.5 and 1.0 μg/kg, respectively. This method can be applied to the quantification of total FF residues in eggs.

Keywords:

• Non-extractable florfenicol residues
• florfenicol amine
• acid-hydrolysis
• ultrahigh-performance liquid chromatography-tandem mass spectrometry

Disclosure statement

We declare that there was no conflict of interest in our paper.

Additional information

Funding

This work was supported by Shandong Key Laboratory of Testing Technique for Food Quality and Safety (Grant No. 2017SYSKFKT03).